Microscopic video showing the disruption of bacteria upon administering a single drop of DEBRICHEM

DEBRICHEM is a disruptive new treatment option to address the infection in hard to heal wounds. The topical gel offers a very effective alternative to surgical debridement, the current standard of care[1].

DEBRICHEM takes an innovative approach to chemical debridement. The acidic gel addresses the biofilm and the pathogens inside the wound bed, that disrupt the onset of the natural healing process[2].

In order to demonstrate the mode of action of our product, we have had tests performed by the Danish Technological Institute. These tests were filmed under a high resulotion microscope in which the bacteria were killed immediately after the application of a single drop of DEBRICHEM. The video can be seen below:

Methods

Bacteria used: Two in-house motile strains were chosen for making the video: Salmo-nella bongorii and Escherichia coli The strains were cultured using Tryptone soy agar (TSA) and Tryptone soy broth (TSB).

Micropore filter biofilm (prepared according to reference [3]): Each bacterial strain was plated on TSA plate and incubated at 37 °C for 24 hours. A single colony from each plate was picked and inoculated into TSB medium and incubated at 37 °C for 24 hours with shaking at 150 RPM. A drop (10μL) of overnight bacteria was transferred to a 0.22 μm polycarbonate filter placed on TSA plate. The plate was incubated at 37 °C for 2 hours before microscopy.

Microscopy: A spinning disk microscope was used for the assignment. The video was made at real-time speed over 7 seconds to show the biofilm followed by adding a few drops of methanesufonic acid (DEBRICHEM).

DEBRICHEM works by withdrawing water from the any type of biological material. Hereby rapidly desiccating, and thus disrupting the biofilm and the pathogens in the wound, ultimately allowing the healing process to start. Want to know more about our product? Visit Our Product page or get in touch with our sales representatives.


[1] Schultz GS et all. Wound bed preparation: a systematic approach to wound management. Wound Repair Regen 2003;11(s1 suppl): S1-S28

[2] Cogo et al, Wounds 2021;33.1-8

[3] Bjarnsholt T, Alhede M, Jensen PØ, et al. Antibiofilm Properties of Acetic Acid. Advances in Wound Care. 2015 Jul;4(7):363-372. DOI: 10.1089/wound.2014.0554.

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